Isolasi bakteri pendegradasi Phenanthrene dari Batanta-Salawati Raja Ampat Papua


Isolation of bacteria degrading Phenanthrene in Batanta-Salawati districts Raja Ampat Papua. Polycyclic aromatic hydroacarbons (PAHs) are important environmental contaminants in soil and water. These compounds have a potential risk to human health, as many of them are carsinogenic and toxic to marine organisms such as diatome, gasthrophode, mussel, and fish. Phenanthrene is one of the hazardous hydrocarbon compounds. The purpose of this research was to characterize microbial strains from Batanta-Salawati Raja Ampat Papua Island and their ability to remove phenanthrene. Two isolates were identified at their physiological characteristics based on salinity tolerance, pH tolerance and the composition of nitrogen base. Molecular identification based on 16S rRNA gene sequences indicated that bacteria had the highest similarity with Rhodobacteraceae bacterium F9 and Roseobacter sp. RW 37. Rhodobacteraceae bacterium F9 could grow optimum on ONR7a media with 5% salinity and at pH of 5-7,5 while Roseobacter sp. RW 37 could grow optimum on ONR7a media with 2% salinity and at pH of 6,2-7,5.

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