Biodiversity of soil microbes from Rhizosphere at Wamena Biological Garden (WBiG), Jayawijaya, Papua

ABSTRAK

The isolation, identification and population of soil microbes from rizosphere at WBiG had been done in the Soil LaboratoriesMicrobiology,Microbiology Division,Research Center ofBiology, Indonesian Institute of Sciences (LIPI), Bogor. The soil was collected randomly from 16sites in WBiG, and taken from 0-15 cm depth.Isolates of microbeswere identified by Bergeys manual method for bacteria; Ellis method forfungi, and the morphology of isolate method for Actinomycetes. The population of microbes was estimated by plate count method.Theresult of isolation, identification and population soil microbes from16samples in WBiG showed that20isolates of bacteria (Azotobactersp., Accinetobactersp. , Bacillussp., Citrobactersp., Flavobacteriumsp., Klebsiellasp., Nitrosomonassp., Pseudomonassp., Rhizobiumsp., Thiobacillussp.,Azospirillumsp., Azotobacter chrococcum, Bacillus panthothenticus, Chromobacterium violaceum, C.lividum,Escherrrichia coli, Flavobacterium breve, Klebsiella aerogenes, Spaerotillus natans,andStaphylococcus epidermidis);nine isolates of fungi(Aspergillus niger,Bisporomyces, Moniliasp.,Cephalosphariumsp.,Verticillumsp., Giocladiumsp., Penicilliumsp., Nelicocephalumsp.,andCuninghamellasp.), and seven isolates of Actinomycetes (Streptomyces, Streptosporangium, Nocardia, Thermomonospora,Thermoactinomyces, Micromonospora, Mycobacterium).The population ofBacillus(108-109),Rhizobium(106-107),Azospirillum(106-107),andThiobacillus(104-109) were founded all of soil samples.